Animal Immunopurification
Introduction
Customers provide expressed proteins, peptide antigens, or small molecule proteins, and our company uses new rapid immunization adjuvants, optimized and validated immunization protocols, or according to customer requirements, selects a short or standard cycle for animal immunization, and finally provides antigen-specific affinity purification of the antibody.AssayVector has completed the immunization and purification of more than 1,000 antigens per year. AssayVector's experience, high potency (over 90% of AssayVector's animal immunization orders are rabbit antibodies with over 1 million potency), reputation, and ability to choose from a variety of immunization hosts are well received by researchers and companies and are key features of our service:
· Proprietary adjuvant to ensure proper mixing of antigen and adjuvant!
· Multiple subcutaneous injections to increase immunogen immunogenicity!
· 5 shots, ELISA potency against immunogen, rabbit antibody up to 1:160,000, mouse antibody up to 1:64,000 or more!
Service Description
Service Steps |
Description |
Period
|
Deliverables | Quotation |
Animal Immunization | 5 vaccinations per host, consisting of an initial vaccination (with complete Fuchs adjuvant) and 4 booster vaccinations (with incomplete Fuchs adjuvant) | 65 days (optional 40 day short cycle) | Rabbit antiserum 60 mL (other hosts separate), 200 μL rabbit preimmunization negative serum (note if required) | info@assayvector.com
|
Potency Test | The company determines ELISA potency by encapsulating the immunogen in an ELISA. | 2days | Project Reports | |
Antibody purification | Antigen affinity purification or Protein A/G purification | 7days | 5-10 mg affinity purified antibody to 10 ml antiserum |
Service Commitment
· Customers are able to detect the target bands after performing endogenous or IP overexpression Western blot assays on small samples (indicating that antibody validation is satisfactory);
· Western blot endogenous or IP overexpression test negative can not meet the experimental needs, indicating that this antibody order validation failed, then Party A does not need to pay the remaining balance of the project;
· Indirect ELISA for antiserum potency, for immunogenic potency, not less than 1:160,000 for rabbit polyantibodies and not less than 1:6,4000 for mouse polyantibodies;
· The client has the right to be kept informed of the progress of the service program and has full ownership of the results.