Renilla Luciferase Rabbit pAb
Cat No.: ATL1043
Size：
| Product Name: | Renilla Luciferase Rabbit pAb |
| Cat No.: | ATL1043 |
| source: | Rabbit |
| reactivity: | Other |
| applications: | WB,IHC,ICC/IF,FC,IP |
| clonality: | Polyclonal |
| recommended dilution: | WB: 1:2000 ICC/IF: 1:200 FC: 1:50 |
| format: | Liquid |
| immunogen: | A synthetic peptide of Renilla Luciferase |
| calculated molecular weight: | 36 kDa |
| observed molecular weight: | 36 kDa |
| genbank accession number: | P27652 |
| gene id (ncbi): | |
| purification method: | Affinity Purification |
| conjugate: | Un-conjugated |
| storage: | Store at -20°C. Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt. |
| synonyms: | Renilla-luciferin 2-monooxygenase; Renilla-type luciferase |
| category: | Primary Ab |
| concentration: | 0.5mg/ml |
| background: | Upon binding the substrate, the enzyme catalyzes an oxygenation, producing a very short-lived hydroperoxide that cyclizes into a dioxetanone structure, which collapses, releasing a CO2 molecule. The spontaneous breakdown of the dioxetanone releases the energy (about 50 kcal/mole) that is necessary to generate the excited state of the coelenteramide product, which is the singlet form of the monoanion. In vivo the product undergoes the process of nonradiative energy transfer to an accessory protein, a green fluorescent protein (GFP), which results in green bioluminescence. In vitro, in the absence of GFP, the product emits blue light. |