Custom Phospho-specific Antibody
Introduction
AssayVector offers site-modified antibody customization service based on years of experience and technology accumulation. AssayVector's modified site-specific antibodies do not recognize unmodified antigens and have high specificity and sensitivity with a guaranteed immunogen potency of not less than 1:160,000; cross-reactivity with unmodified peptides is <10% of the customized antibodies. A wide range of modified antibody services are available, including:
· Phosphorylation-modified specific antibody preparation
· Methylation modification specific antibody preparation
· Acetylation modification specific antibody preparation
· Succinylation modification specific antibody preparation
· Generation of ubiquitination-modified specific antibodies
· Palmitic acidification modification specific antibody preparation
AssayVector also offers a line of post-translational modification pan-antibodies: lysine acetylation, methylation, succinylation and malondialdehyde-formaldehyde pan-modification specific antibodies.
Service Features
· The peptides were synthesized using professional antigen design software for the identified sites, combined with the literature and extensive selection of peptide sequences with good immunogenicity
· Provide high quality peptide antigens, peptide antigens for immunization are guaranteed to be >90% pure with strict quality control
· Mature experience in peptide coupling technology, selecting good coupling solutions from a variety of carrier proteins and different coupling methods, with a coupling rate >80%
· Use of proprietary adjuvants to ensure good mixing of antigen and adjuvant, and multi-point subcutaneous injection to maximize immunogenic potency
· Immunization of 4 rabbits (to avoid differences in immunization effects caused by differences in animal organisms, increase to 4 experimental animals), preferably 2 rabbits with high potency for purification
Service Description
Service Steps |
Description |
Period
|
Deliverables | Quotation |
Antigen Analysis and Design | According to the modification site specified by the customer, we analyze the immunogenicity, hydrophilicity and homology of the protein to select the appropriate specific peptide sequence. | 2days | Analysis Report | info@assayvector.com
|
Antigen Preparation | Modified peptides were designed and synthesized for specific protein binding site sequences and coupled with KLH for immunization. | 3-4weeks | 1mg modified peptide | |
The corresponding unmodified peptides were synthesized and coupled to BSA for purification. | 1mg unmodified peptide | |||
Animal Immunization | Immunization of 4 rabbits with 5 immunizations per host, including an initial immunization (with complete Fuchs adjuvant) and 4 booster immunizations (with incomplete Fuchs adjuvant). | 65days | Rabbit serum 60 mL (other hosts separate) | |
Potency Test | Detection of serum ELISA potency, encapsulation of immunogens for ELISA detection | 2days | Potency Test Results | |
Antibody purification | Cross-adsorption purification with modified and unmodified peptides | 7days | 5mg affinity purified antibody to 10ml antiserum | |
Potency Test | Detection of antibody ELISA potency, the immunogen, the adsorbed antigen are separately encapsulated in ELISA, the test to check the adsorption effect. | 2days | Potency Test Results Project Report |