LIM kinase 2 Rabbit pAb
Cat No.: APA4586
Size：
| Product Name: | LIM kinase 2 Rabbit pAb |
| Cat No.: | APA4586 |
| source: | Rabbit |
| reactivity: | Human, Mouse, Rat |
| applications: | WB,IHC,ICC/IF,FC,IP |
| clonality: | Polyclonal |
| recommended dilution: | WB: 1:1000 IHC: 1:20 ICC/IF: 1:20-1:50 FC: 1:20 IP: 1:20 |
| format: | Liquid |
| isotype: | IgG |
| immunogen: | A synthetic peptide of human LIM kinase 2 |
| calculated molecular weight: | 72 kDa |
| observed molecular weight: | 72 kDa |
| genbank accession number: | P53671 |
| gene id (ncbi): | 3985 |
| purification method: | Affinity Purification |
| conjugate: | Un-conjugated |
| storage: | Store at -20°C. Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt. |
| synonyms: | LIMK2 |
| category: | Primary Ab |
| concentration: | 0.5mg/ml |
| background: | There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |