SMURF 2 Rabbit pAb
Cat No.: APA4354
Size:
Product Name: | SMURF 2 Rabbit pAb |
Cat No.: | APA4354 |
source: | Rabbit |
reactivity: | Human, Mouse, Rat |
applications: | WB |
clonality: | Polyclonal |
recommended dilution: | WB: 1:1000 |
format: | Liquid |
isotype: | IgG |
immunogen: | A synthetic peptide of human SMURF 2 |
calculated molecular weight: | 86 kDa |
observed molecular weight: | 86 kDa |
genbank accession number: | Q9HAU4 |
gene id (ncbi): | 64750 |
purification method: | Affinity Purification |
conjugate: | Un-conjugated |
storage: | Store at -20°C. Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt. |
synonyms: | SMURF2 |
category: | Primary Ab |
concentration: | 0.5mg/ml |
background: | E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level. |