RAG2 Rabbit pAb
Cat No.: APA3573
Size：
| Product Name: | RAG2 Rabbit pAb |
| Cat No.: | APA3573 |
| source: | Rabbit |
| reactivity: | Mouse |
| applications: | WB |
| clonality: | Polyclonal |
| recommended dilution: | WB: 1:1000-1:5000 |
| format: | Liquid |
| isotype: | IgG |
| immunogen: | A synthetic peptide of mouse RAG2 |
| calculated molecular weight: | 59 kDa |
| observed molecular weight: | 59 kDa |
| genbank accession number: | P21784 |
| gene id (ncbi): | 19374 |
| purification method: | Affinity Purification |
| conjugate: | Un-conjugated |
| storage: | Store at -20°C. Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt. |
| synonyms: | Rag-2 |
| category: | Primary Ab |
| concentration: | 0.5mg/ml |
| background: | Core component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. DNA cleavage by the RAG complex occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3---hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5---phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at --Lys-4-- (H3K4me3) stimulates both the nicking and haipinning steps. |